LAG-3 expression on CD4+ and CD8+ T cell subsets. (A) Representative ex vivo surface staining of LAG-3 on CD4+ and CD8+ T cells. (B) LAG-3 expression on both CD4+ and CD8+ T cells among HIV-N, HIV+ and HIV+ ART groups. (C) Correlation between LAG-3 expression and CD4 count among HIV+ participants. (D) Correlation and (E) co-expression of LAG-3 and CD69 among all HIV+ participants. Statistical comparisons made by Kruskal-Wallis (with Dunn’s post-test), Wilcoxon test and Spearman correlation. *p < 0.05, ****p < 0.0001.