HIV-1 non-macrophage-tropic R5 envelope glycoproteins are not more tropic for entry into primary CD4+ T-cells than envelopes highly adapted for macrophages

Table 1 T/F, acute, late stage non-mac-tropic and late stage mac-tropic R5 Envs

Patient	Clade	Non-mac-tropic			Mac-tropic			Reference
Patient		Env	Origin	Full ID	Env	Origin	Full ID	Reference
AIDS patients
NA20	B	LN8	LN¹		B59	FL²		[3]
					B501	FL
NA420	B	LN40	LN		B33	FL		[3]
JR	B	JR-CSF	CSF³		JR-FL	FL		[4]
P1114	B	98-27	Plasma		98-15	Plasma		[3]
P7766	B	SP1	Spleen	SP13-33-41	FL2	FL	FL19-56-66	[23]
P10017	B	SP2	Spleen	SP10-9-65	FL1	FL	FL9-1-2	[23]
P6568	B	SP1	Spleen	SP6-11-9	FL1	FL	FL11-1-249	[23]
CA110	B	SP2	Spleen	SP53-23-131	OC1	OL⁴	OC58-11-57	[23]
		SP3 SP4	Spleen Spleen	SP53-6-122 SP52-16-50	SP1	Spleen	SP52-13-34
T/F
3 T	B	3 T	Plasma	p1054.TC4.1499				[60]
6 T	B	6 T	Plasma	p63358.p3.4013				[60]
15 T	B	15 T	Plasma	p700010040.C9.4520				[60]
19 T	B	19 T	Plasma	pPRB958_06.TB1.4305				[60]
Acute Stage
R463F	A1	R463F	Plasma	R463FPL16MAR07EnvE44				[62]
R880F	A1	R880F	Plasma	R880FPL12JAN07EnvA6				[63]
Z185F	C	Z185F	Blood	Z185FPB24AUG02ENV3.1				[63,64]
Z205F	C	Z205F	Blood	Z205FPB27MAR03ENV1.1				[63,64]
R66M	A/C	R66M	Plasma	R66MPL7MAR06.3A9Env				[63]
Z1792M	C	Z1792M	Plasma	Z1792MPL18DEC07.3G7Env2				[63]
Z201M	C	Z201M	Plasma	Z201MPL7FEB03ENV2.1				[13]
Z221M	C	Z221M	Plasma	Z221MPL7MAR03ENV2.1				[13]
Z153M	C	Z153M	Plasma	Z153MPL13MAR02ENV5.1				[13]

We selected pairs of highly mac-tropic and non-mac-tropic R5 Envs from 8 subtype B infected AIDS patients (including JR-FL and JR-CSF) for study. For subject NA20, we included 2 highly mac-tropic Envs. For CA110, we included two highly mac-tropic Envs and two non-mac-tropic spleen Envs. Except for JR-FL and JR-CSF, all Envs had been PCR amplified directly from patient tissues. We also included 13 diverse R5 Envs previously reported as T/F or isolated from the acute stage of infection. In this group, we included Envs from different subtypes. 1. Lymph node; 2. Frontal lobe; 3. Cerebral spinal fluid; 4. Occipital lobe.

ISSN: 1742-4690