Env+ pseudovirion infection of HeLa TZM-bl and macrophages. (A) Macrophage-tropic R5 Envs mediate 100-1000-fold higher infection of macrophages compared to T/F/acute and late disease non-mac-tropic R5 Envs. (B) Several late disease non-mac-tropic Envs and most T/F/acute Envs mediated lower infection of HeLa TZM-bl cells compared to macrophage tropic Envs. (C) Plotting macrophage infectivity titers as a percent of TZM-bl titers suggests that T/F/acute Envs are not as non-mac-tropic as late disease non-mac-tropic Envs. Significant differences were evaluated using Mann Whitney or Wilcoxon matched pair tests as described in Materials and Methods. Pseudoviruses carrying clade B Envs are represented by black symbols, those with clade C Envs by light gray symbols, clade A by hatched symbols and CRF A/C by dark gray. Median values are marked.