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Figure 6 | Retrovirology

Figure 6

From: Myocyte enhancer factor (MEF)-2 plays essential roles in T-cell transformation associated with HTLV-1 infection by stabilizing complex between Tax and CREB

Figure 6

HTLV-1 LTR transcriptional activation complexes contain MEF-2. Binding of various transcription factors present in the nuclear extract isolated from MT-2 cells to the HTLV-1 LTR was assessed using the Promoter-Binding Transcription Factor Profiling Array I upon knocking down MEF-2A (A) and Tax (B) as described in Methods. The nuclear extract was incubated with oligonucleotide probe mix with the HTLV-1 LTR or a control DNA. The binding of each transcription factor to LTR was indicated by average reduction in chemiluminescence of transcription factor-specific oligonucleotide probe specific to each factor from triplicate samples.

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