Figure 6

Sense and antisense transcripts of mouse Rtl1/Mart1 , Rgag4/Mart5 , Ldoc1l/Mart6 , Ldoc1/Mart7 and Cxx1a,b,c/Mart8b,c,a in placenta tissue at E14.5 using TAG-aided sense/antisense transcript detection (TASA-TD). (A) Schematic drawing of the methodology used for first strand cDNA synthesis with a gene and strand specific TAG labeled primer (GSP sense/antisense (RT) TAG) and the following PCR with another gene and strand specific primer (GSP sense/antisense (PCR)) and a TAG-primer) (B) First strand cDNA synthesis and PCR identification as well as quantification of sense (s) and antisense (as) transcripts of Rtl1/Mart1, Rgag4/Mart5, Ldoc1l/Mart6, Ldoc1/Mart7, Cxx1c/Mart8a and Cxx1a,b/Mart8b,c. Beta-actin was used as control.