Figure 5

ISH localization at E8.5 and E15.5 and PCR of the three different Mart8a, −b and -c gene loci in mouse placenta. (A) ISH identifying Cxx1c/Mart8a antisense and Cxx1a,b/Mart8b,c sense transcripts (left panels) and Cxx1b/Mart8a sense and Cxx1a,b/Mart8b,c antisense transcripts (right panels) at E8.5 and E15.5. Black arrows identify positive pTGCs from congruent low (top) and high (bottom) magnified regions in both pictures. Bars are shown in μm. For abbreviations see Figure 3. (B) PCR of Cxx1c/Mart8a and Cxx1a,b/Mart8b,c sense (s) and anti-sense (as)transcripts at E8.5, E12.5, E14.5, E16.5 and E18.5 using specific primers with placental cDNA. Beta-actin was used as positive control. (C) Schematic drawing of the mouse Cxx1/Mart8 genes and two additional genes Gm14592, Gm14600 on chromosome X A5 according to the current assembly: GRCm38.p2. Cxx1a/Mart8b and Cxx1b/Mart8c (red arrows) are localized on the opposite DNA strand thus, in the opposite orientation in context to Cxx1c/Mart8a (green arrow). All DNA regions and the length of each Mart gene are signified in base pairs. (D) Northern analyses identifying Cxx1c/Mart8a antisense and Cxx1a,b/Mart8b,c sense transcripts (left lanes) and Cxx1b/Mart8a sense and Cxx1a,b/Mart8b,c antisense transcripts (right lanes) at E12.5 and E14.5.