Figure 3

Localization of Rgag4/Mart5 and Ldoc1l/Mart6 in the mouse placenta at E8.5 and E14.5 by ISH. (A) Schematic drawing shows an overview of the structure of the mouse placenta at E15.5 with decidua cells (DC) in the decidua (d); junctional zone (jz) containing parietal trophoblast giant cells (pTGC); glycogen trophoblasts (GlyT) and spongiotrophoblasts (ST) and the labyrinth (l) containing sinusoidal trophoblast giant cells (sTGC) and syncytiotrophoblast-I/-II (SCT-I, SCT-II). Two right pictures show Periodic acid-Shiff (PAS) stained placenta tissue sections congruent with tissue layers and cells identified in the left drawing. Darkly stained purple PAS positive cells identify glycogen trophoblasts (GlyT). Also shown are negatively stained spongiotrophoblasts (ST) and parietal trophoblast giant cells (white arrow). (B) In situ hybridization identifying Rgag4/Mart5 and Ldoc1l/Mart6 sense and antisense transcripts at E8.5 and E14.5. For each transcript detected, the left and right panels with black arrows pointing to pTGC identify low and high magnifications of the same regions. White arrows show pTGC in different magnified regions of the same tissue. Bars are shown in μm. Decidua (d), junctional zone (jz), labryrinth (l), EPC, ectoplacental cone; CH, chorion; spongiotrophoblast (ST); glycogen trophoblast (GlyT).