Ecotopic expression in JR5 cells and infection assays. (A). A NIr immunoblot of cell lysate samples is presented with TRIM5α signal in red and actin in green. Samples are identified above their respective lanes, with molecular mass standard sizes and band identities displayed at the margins. (B). A graph of fluorescent immunoblot signal intensities in the NIr blot relative to those of the untransduced JR5 cells is presented with the amount of endogenous, human TRIM5α-colored blue, xenogeneic TRIM5α colored yellow, and co-migrating TRIM5α proteins colored green. (C) A graph of flow cytometry analysis results for GFP signal from single-round infectivity assays (MOI = 0.05) using HIV-1NL4-3 and SIVmac239-expressing GFP vectors relative to the values for the JR5 positive controls is presented. (D) A graph of results of flow cytometry analysis for intracellular CA 40-h post infection with either HIV-1NL4-3 or SIVmac239 (MOI = 0.05) is presented. Graphs C and D are averages of three independent experiments and error bars indicate standard deviation. Statistical of the experimental versus the control values for the data in both panels C and D generated p-values of <0.012 for all pairs except for the rhTRIM5α/SIV sample, p = 0.37.