Transcriptome analysis of the effect of CCL2 neutralization. MDM were treated or not with anti-CCL2 Ab (2.5 μg/ml) for 20 h. Total RNA was then extracted, subjected to poly(A) selection followed by reverse transcription, generation of cDNA libraries, and sequencing. (A) Dotplot of DESeq2 normalized counts of the A3A gene following 20 h exposure to anti-CCL2 Ab. Each condition has two biological replicates collected from two donors. Dots were colored by donors. (B) Heatmap of 24 defense-response-to-virus genes which are upregulated two-fold in 20 h anti-CCL2 Ab versus the 20 h control MDM. Heatmap were made by GENE-E (http://www.broadinstitute.org/cancer/software/GENE-E).