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Figure 6 | Retrovirology

Figure 6

From: Endogenous CCL2 neutralization restricts HIV-1 replication in primary human macrophages by inhibiting viral DNA accumulation

Figure 6

Effect of the timing of anti-CCL2 Ab addition on HIV-1 replication and A3A expression. MDM were treated with anti-CCL2 or control Ab (2.5 μg/ml) 20 h before or at the time of infection with HIV-1BaL (3000 TCID50 per well) as described in Methods. (A) Total DNA was extracted 7 days after infection and the amount of total HIV-1 DNA (copies/106 cells) was determined by qPCR. Data represent mean values (+SE) of the results obtained with 3 different donors. (B) Cells were recovered 14 days after infection and HIV-1 Gag expression was evaluated by flow cytometry. Data represent mean values (+SE) of the results obtained with 7 different donors. *p < 0.05; ***p < 0.001. (C) Cells were lysed 14 days after infection, and A3A protein expression in whole cell extracts was detected by western blot. Actin was used as house-keeping control. The ratio of A3A to actin protein was determined by densitometry, and graph shows the fold relative to control. The result from one representative experiment of 4 independently performed is shown.

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