Figure 1

Neutralization of endogenous CCL2 reduces the proportion of HIV-1 infected MDM. MDM were treated with anti-CCL2 or control Ab (2.5 μg/ml) for 20 h. (A, B) Cells were then infected with HIV-1_BaL (3000 TCID₅₀ per well) as described in Methods. After 14 days, cells were recovered and HIV-1 Gag expression was evaluated by flow cytometry. Values indicate the percentage of p24 Gag⁺ cells for each sample. In A, the results from 3 representative donors out of 22 tested are shown. In B, data represent mean values (+SE) of the results obtained with all the donors analyzed. ***p < 0.001 (anti-CCL2 Ab vs. control Ab). (C) Cells were challenged with (VSV-G) HIV-1 (250 ng CAp24 equivalent per 10⁵ cells) in a single-round infectivity assay. Some cultures were also treated with AZT (10 μM). The percentage of p24 Gag⁺ cells was assessed by flow cytometry 3 days after infection. Data represent mean values (+SE) of the results obtained with MDM from 4 different donors. *p < 0.05 (anti-CCL2 Ab and AZT vs. nil).