Figure 3

HIV-2 infection of monocyte-derived dendritic cells (MDDCs). (a) Infection of MDDCs by primary HIV-2. MDDCs were exposed to HIV-2 ROD, GL-AN (150 ng p27 mL-1) and GL-AN (VSV) (50 ng p27 mL-1). After 3 days, the levels of Gag and SAMHD1 were measured by flow cytometry. A representative experiment is depicted. (b) Infection with HIV-2 ROD, TOE (150 ng p27 mL-1) and ROK (100 ng p27 mL-1). After 3 days, the levels of Gag, SAMHD1 and MxA were measured by flow cytometry. Data are Mean of 6 independent donors. The innate response was analyzed by measuring by flow cytometry the levels of the interferon-stimulated gene MxA. (c) Infection of MDDCs by HIV-2 GL-AN expressing or not Vpx. MDDCs were exposed to HIV-2 GL-AN WT and GL-AN ∆Vpx (150 ng p27 mL-1) and analyzed as described in (b). (d) Infection of MDDCs with HIV-2 GL-AN WT or ∆Vpx virions pseudotyped with VSV-G. MDDCs were exposed to HIV-2 GL-AN (VSV) WT and GL-AN (VSV) ΔVpx (50 ng p27 mL-1) and analyzed as described in (b).