Proliferation of FV-specific CD8 +T cells in vitro . TLR3+/+ and TLR3−/− mice were infected with 20,000 SFFU of FV. At 4 dpi bone marrow cells were isolated and cultured for 7 days to obtain BM-DCs. BM-DCs were loaded with FV GagL peptide and co-cultured with CFSE-labeled FV-specific CD8+ T cells for 3 days. Proliferation of transgenic CD8+ T cells was determined by flow cytometry by loss of CFSE dye as calculated numbers of proliferating cells (A) or a representative histogram showing the amount of cells in each generation (B). A minimum of six mice were used for analysis. At least two independent experiments were performed. Significant differences between the groups are indicated by * for p < 0.05.