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Figure 3 | Retrovirology

Figure 3

From: Friend retrovirus drives cytotoxic effectors through Toll-like receptor 3

Figure 3

Effector functions of NK cells. TLR3+/+ and TLR3−/− mice were infected with 20,000 SFFU of FV. At 4 dpi numbers of NK cells (CD3 CD49b+ NK1.1+) in the spleen of FV-infected or naive mice were analyzed by flow cytometry (A). At least six mice of minimum two independent experiments were used and the mean values are shown by bars and dots + SEM. The cytotoxic potential of splenic NK cells was analyzed in an in vitro NK cell cytotoxicity assay. NK cells were isolated from spleens of FV-infected TLR3+/+ and TLR3−/− mice (B). FV-derived tumor cells (FBL-3) were stained with CFSE and co-cultured with isolated NK cells at different effector-target ratio of 50:1 to 6:1 for 24 h. At least four mice were used for the analysis. Statistically significant differences between the groups of infected TLR3+/+ and TLR3−/− mice are indicated by * for p < 0.05.

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