Phenotypic analysis of splenic DCs in FV-infected mice. TLR3+/+ and TLR3−/− mice were infected with 20,000 SFFU of FV. At 4 dpi splenic DCs were isolated and analyzed for Tlr3 mRNA expression (A) and mRNA expression of Pkr, Ifi44, Ifn-a4 and Ifn-b (B). Splenic DCs (CD11c+ CD11b+) were also analyzed by flow cytometry. Surface expression of the costimulatory molecule CD86 (C, D) and MHC-II (E, F) were determined. Uninfected TLR3+/+ and TLR3−/− mice were used as controls (white bars). A minimum of nine mice per FV-infected group were analyzed and the individual values are indicated by bars and dots + SEM. Experiments were repeated at least twice. Statistically significant differences between the groups are indicated by * for p < 0.05, ** for p < 0.01 and *** for p < 0.001.