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Figure 1 | Retrovirology

Figure 1

From: Detailed characterization of antibody responses against HIV-1 group M consensus gp120 in rabbits

Figure 1

Identification of immunogenic linear epitopes by ELISA using overlapping peptides. ELISA was conducted with antisera collected from three rabbits after the second, fourth and fifth immunizations using overlapping peptides. Peptide numbers represent catalog numbers from the NIH AIDS Reagent Program. A schematic diagram of gp120, as well as gp120-OD, is shown on top. Corresponding regions on the eOD-GT6 construct and identical amino acids are shown in green bars. MCON-S sequence, from which peptides are derived, is shown. Amino acid residues that differ on MCON6, from which the immunogen is generated, are indicated with small red squares. Sequences of MCON6-specific peptides (V1, V2, V4 and V5) are indicated in red text at the top. Blue circles below the schematic diagram of gp120 indicate amino acid residues that make contact with CD4 and/or VRC01. A450 represents absorbance value at 450 nm.

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