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Table 1 qHRG missense mutation experimental validation

From: High-throughput profiling of point mutations across the HIV-1 genome

HIV-1 genomic region Substitution DNA mutation DNA coverage a RC index qPCR value b
Capsid A194T G1765A 216 0.189 0.421
Protease D25G A2326G 165 0.068 0.002c
Protease D29G A2338G 178 0.151 0.241
Reverse transcriptase E6K G2565A 353 0.527 0.849
Reverse transcriptase F61S T2731C 150 0.079 0.037
Reverse transcriptase Y501C A4051G 190 0.04 0.037
Integrase N155Y A4692T 90 0.119 0.002c
vif D101N G5341A 223 0.453 0.981
rev E10G A5997G 292 0.698 1.714
gp120 C119G T6575G 123 0.082 0.021
gp120 K205M A6834T 337 0.169 0.123
gp120 D476V A7635T 383 0.024 0.026
gp40 Y136H T8126C 206 0.137 0.012c
3’-LTR N/A C9547T 234 0.18 0.132c
  1. aNumber of mutation initial sequence counts.
  2. bValue obtained from [ng RNA mutant]/[ng RNA WT].
  3. cFor mutants where [ng RNA] < 0.01, ELISA measured [ng p24 mutant]/[ng p24 WT] ratios were averaged into value reported.