High-throughput profiling of point mutations across the HIV-1 genome

Retrovirology

Table 1 qHRG missense mutation experimental validation

HIV-1 genomic region	Substitution	DNA mutation	DNA coverage ^a	RC index	qPCR value ^b
Capsid	A194T	G1765A	216	0.189	0.421
Protease	D25G	A2326G	165	0.068	0.002^c
Protease	D29G	A2338G	178	0.151	0.241
Reverse transcriptase	E6K	G2565A	353	0.527	0.849
Reverse transcriptase	F61S	T2731C	150	0.079	0.037
Reverse transcriptase	Y501C	A4051G	190	0.04	0.037
Integrase	N155Y	A4692T	90	0.119	0.002^c
vif	D101N	G5341A	223	0.453	0.981
rev	E10G	A5997G	292	0.698	1.714
gp120	C119G	T6575G	123	0.082	0.021
gp120	K205M	A6834T	337	0.169	0.123
gp120	D476V	A7635T	383	0.024	0.026
gp40	Y136H	T8126C	206	0.137	0.012^c
3’-LTR	N/A	C9547T	234	0.18	0.132^c

^aNumber of mutation initial sequence counts.
^bValue obtained from [ng RNA mutant]/[ng RNA WT].
^cFor mutants where [ng RNA] < 0.01, ELISA measured [ng p24 mutant]/[ng p24 WT] ratios were averaged into value reported.

ISSN: 1742-4690