Figure 4
From: A HIV-1 Tat mutant protein disrupts HIV-1 Rev function by targeting the DEAD-box RNA helicase DDX1
Nullbasic alters the subcellular localization of DDX1. A. HeLa cells were transfected with empty vector alone (pcDNA3.1, row 1), MYC-Rev alone (row 2), Nullbasic (NB)-mCherry alone (row 3 a) or MYC-Rev with NB-mCherry (row 4). Fixed cells were immunostained with anti-MYC (green) and anti-DDX1 (magenta, upper panels) and were visualized alone with NB-mCherry (red) by fluorescence microscopy. Nuclei were stained with DAPI. The overlay panels show the merge of the Rev panel with DDX1 (row 1, 2, and 4) and the NB-mCherry panel with DDX1 panel (row 3). Images are representative of at least five fields selected randomly from three independent experiments. B. Quantification of nuclear/cytoplasmic fluorescence ratios(Fn/c) for DDX1as described in Methods. Mean of Fn/c ± SD were calculated from 60 HeLa cells from 3 independent experiments.