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Figure 4 | Retrovirology

Figure 4

From: Genome-wide shRNA screening identifies host factors involved in early endocytic events for HIV-1-induced CD4 down-regulation

Figure 4

Knock-down of some genes involved in CD4 down-regulation decreases replication of HIV-1 in SupT1 cells. A. Knock-down of four genes involved in CD4 down-regulation (EPS15, DNM3, ATP6AP1, IDH3G) decreases HIV-1 replication: graphs show levels of HIV-1 infection at different time points (2, 4 and 7 days post-infection), as measured by the percentage of eGFP expressing cells in the population. The grey lines represent HIV-1 replication in the negative control (cells expressing the non-targeting shRNA), the black line represents cells expressing the target shRNAs. Bar graphs represent the standard deviations for 5 to 6 independent experiments (p values: *p ≤ 0.03, **p ≤0.008). B. Knock-down of HRBL, SNX22, HSP90B1 does not affect HIV-1 replication: graph represent levels of HIV-1 infection as described for Figure 4A.

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