Figure 7
Competition between Tax 11–19 and HBZ 26–34. (A) Tax11–19 inhibits presentation of HBZ26–34 under certain conditions. CFSE-labelled BLCL were incubated with a range of concentrations of Tax11–19 peptide for 1 h. HBZ26–34 peptide was subsequently added. After a further 1 h culture, HBZ-1 CTLs were added at an E:T ratio of 3:1. After 6 h incubation, cells were fixed, and the absolute number of CFSE+ cells present in each culture condition was determined by flow cytometry. These data are representative of two experiments performed in duplicate. (B) Recall CTL responses to HBZ26–34 and Tax11–19. PBMC depleted of CD4+ T cells were cultured either alone, or in the presence of 2 uM Tax11–19 or HBZ26–34. The frequency of IFN-γ producing cells was enumerated by Elispot. AC are represented by circular symbols; P, square symbols and HAM/TSP, triangular symbols.