Figure 5

Flow cytometric quantitation of the histone acetylation levels in lymphocytes from different phases in the two groups of monkeys. (A) Lymphocytes gated by size on a forward scatter vs. side scatter plot. (B) Two monkeys were selected from different groups for representative flow cytometric analyses of different histone acetylation levels. The acetylated histone levels of the gated cells were analyzed by measuring the fluorescence intensity of these cells. The figure presents the difference in the lymphocytes’ acetylation levels in the different groups at week 54 (5 weeks after Pc infection). (C) Higher histone acetylation levels were observed in lymphocytes in the ART + Pc group during Pc infection (MFI of 188.90 ± 51.33 at week 54 vs. 125.96 ± 20.80 at week 57). MFI values from weeks 54 and 57 in the same group were combined to examine the difference in acetylated histone levels between the two groups during the malaria phase. The Mann–Whitney U test was utilized for statistical analyses. The data are shown as the mean ± SD.