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Table 2 Human IgA isolation methods

From: Are anti-HIV IgAs good guys or bad guys?

Reagent for IgA isolation

IgA site recognized

IgA form recognized

References

Jacalin resin

α-D-galactose (O-linked) in the IgA1 hinge

Preferential binding to all IgA1 forms; Weak binding to IgA2

[33],[34],[37]

Staphylococcal superantigen-like protein 7

Fc

Specific for all IgA1 and IgA2 forms

[36],[39]

Streptococcal IgA-binding peptide

Fc

Specific for all IgA1 and IgA2 forms

[35],[38]

mAbs

   

Anti-J chain mAb

J chain

Specific for dimeric and secretory forms of IgA1 and IgA2

Commercially available

Anti-SC mAb

SC

Specific for secretory forms of IgA1 and IgA2

Specific anti-IgA1 mAb

Constant region

Specific for IgA1

Specific anti-IgA2 mAb

Constant region

Specific for IgA2