HIV-2 interaction with cell coreceptors: amino acids within the V1/V2 region of viral envelope are determinant for CCR8, CCR5 and CXCR4 usage

Table 3 Primers used in site-directed mutagenesis of V1V2 region of HIV-2 _MJC97 surface envelope glycoprotein

Primers	Mutated residues*	Orientation	Sequence (5′ to 3′)**	Location***
V1V2 Mut1	K98N, S99I	+	GAGTTGTAACAACATAAGTGAAA	282–304
V1V2 Mut1-R	K98N, S99I	-	TTTCACTTATGTTGTTACAACTC	282–304
V1V2 Mut2	N104T, S106T	+	AAACCACAACAACCACAAGTAACAAC	302–327
V1V2 Mut2-R	N104T, S106T	-	GTTGTTACTTGTGGTTGTTGTGGTTT	302–327
V1V2 Mut3	M147N	+	GTCAGTTCAACATGACAGGG	431–450
V1V2 Mut3-R	M147N	-	CCCTGTCATGTTGAACTGAC	431–450
V1V2 Mut4	Q160N	+	AAATCATATAACGAAACAT	469–487
V1V2 Mut4-R	Q160N	-	ATGTTTCGTTATATGATTT	469–487
V1V2 Mut5	S114P, T116D, D117Q	+	ATCTATCCCCACAGACCAGTACAGC	333–357
V1V2 Mut5-R	S114P, T116D, D117Q	-	GCTGTACTGGTCTGTGGGGATAGAT	333–357
V1V2 Mut6	Y118E, S119Q, L120E	+	GACCAGGAGCAAGAGATAAATGAGAGTTCTCC	346–377
V1V2 Mut6-R	Y118E, S119Q, L120E	-	GGAGAACTCTCATTTATCTCTTGCTCCTGGTC	346–377
V1V2 Mut7	P172T, T173N, T176S	+	GTATGTGAAACAAATAATGAAAGCACAAGCA	505–535
V1V2 Mut7-R	P172T, T173N, T176S	-	TGCTTGTGCTTTCATTATTTGTTTCACATAC	505–535
V1V2 Mut5′	I113P, S114G, T115S	+	ACAACATCTCCAGGGAGCACA	328–351
V1V2 Mut5′-R	I113P, S114G, T115S	-	TGTGCTCCCTGGAGATGTTGT	328–351
V1V2 Mut6′	D117L, Y118K, S119P	+	ACACTCAAACCCTTGATAAATGAGA	346–370
V1V2 Mut6′-R	D117L, Y118K, S119P	-	TCTCATTTATCAAGGGTTTGAGTGT	346–370
V1V2 Mut7′	T173F, E175T	+	TGTGAACCATTTAATACCACAACAAGC	508–534
V1V2 Mut7′-R	T173F, E175T	-	GCTTGTTGTGGTATTAAATGGTTCACA	508–534

*and ***Numbers of amino acid residues or nucleotides are referred to HIV-2_MJC97 sequence (GenBank accession number: EU021092). **Mutations in the primer sequence are represented in boldface.

ISSN: 1742-4690