Figure 3
Amino acid residues changed in V1/V2 region of env glycoprotein by site-directed mutagenesis. Amino acids are denoted by single-letter code. (A) For a better localization of mutated amino acids, the sequence alignment between HIV-2ROD (GenBank accession number: M15390) and HIV-2MJC97 (GenBank accession number: EU021092) was included. The red boxes indicate the conserved regions between HIV-2ROD and HIV-2MJC97 amino acids sequences. (B) The first set of mutants (MJC97mt1 to MJC97mt7) was obtained by sequential mutagenesis starting in the non-mutated recombinant virus, MJC97wt. The second set of mutants (MJC97mt5′ to MJC97mt7′) was derived from the V1/V2 of MJC97mt4. For each sequential mutant, underlined red letters represents the newly changed amino acids residues, while the non-underlined red letters denote mutations that were previously added. Amino acids residues (in panel A and B) were numbered according to HIV-2ROD (GenBank accession number: M15390) or HIV-2MJC97 sequence (GenBank accession number: EU021092).