HIV-1 virion production is dependent on glycolysis. A. Infectivity of culture supernatants from HIV-1 NL4.3 infected primary CD4+ T cells, Jurkat (Jur) and CEM-ss (CEM) cells that were cultured for 24 hours in media containing 1 g/L glucose or galactose, was determined by β-galactosidase activity of the indicator cell line TZM-bl. B. Western blots showing the presence of p24Gag in the supernatant from cultures of HIV-1 NL4.3 infected primary CD4+ T cells , Jurkat and CEM-ss in cultures containing 1 g/L galactose (gal) or glucose (glc). C. Quantified data from western blotting of p24Gag shown in (B). All experiments were performed in triplicate and are shown as the average normalised to the infectivity of cultures containing glucose. Error bars represent the standard deviation.