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Figure 4 | Retrovirology

Figure 4

From: HIV-1 pathogenicity and virion production are dependent on the metabolic phenotype of activated CD4+ T cells

Figure 4

Cells infected with HIV-1 NL4.3 have a survival advantage when cultured in media containing galactose compared with glucose. A. Primary CD4+ T cells were infected with HIV-1 NL4.3 in RPMI with IL-2. After 24 hours cells were washed and seeded into DMEM containing galactose, glucose or a combination of the two in the absence or presence of reverse transcriptase inhibitors (RTI). Cells were harvested at 24 hour intervals and analysed for intracellular HIV-1 p24Gag expression by flow cytometry. Data are shown as the percentage of p24Gag positive cells. B. The percentage of p24Gag positive Jurkat and CEM-ss cells cultured in DMEM containing galactose, glucose or a combination of the two in the absence or presence of reverse transcriptase inhibitors (RTI). C. Comparison of the percentage of p24Gag positive primary CD4+ T cells in cultures containing galactose (gal) or glucose (glc) from five donors for each day in the time course experiment, with the associated p-values as calculated with a paired t-test.

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