Monitoring glycolysis and oxidative phosphorylation in HIV-1 NL4.3 infected and uninfected CD4+ T cells upon provision of glucose. A. The extracellular acidification rate (ECAR) and B. oxygen consumption rate (OCR) over time is given as a percentage of the baseline recorded at 41 minutes in the absence of glucose for primary CD4+ T cells from three donors, CEM-ss and Jurkat cells. Vertical lines indicate the injection of glucose and oligomycin, respectively. Data from primary CD4+ T cells represent single plates with 10 wells dedicated to each condition and data for cell lines CEM-ss and Jurkat are the average of two plates. In all cases, infected cell populations were at least 70% positive for HIV-1 p24Gag as determined by flow cytometry. Error bars represent the standard deviation and asterisks indicate p-values from an unpaired t-test: *0.01 < p < 0.05, **0.005 < p < 0.01, ***p < 0.005, data points without an asterisk have non-significant differences.