Figure 3
Role of pal II and PBS pal in MMTV gRNA dimerization, RNA packaging and viral propagation. (A) Description of the pal II mutants. (B) Typical RNA dimerization in TBM and TB gels of wild type and mutant MMTV RNAs. M: monomer lane or monomer conformer; D: dimer lane or dimer conformer. (C) Quantification of the relative RNA dimerization levels (see table below the histogram). Table above the histogram represent the P values of the respective mutants in TBM and TB gels. The experiments were repeated 2-6 times. (D) Relative packaging efficiency of transfer vector RNAs. (E) RNA propagation efficiency expressed as Hygr colony forming unit per ml (CFU/ml). In (C), (D), and (E), the histograms represent data from at least three independent experiments (± SD). The P values for all mutants in panels (D) and (E) were significant (<0.001).