Figure 1

Predicted and SHAPE-validated structural models of the MMTV packaging signal RNA. (A) MMTV packaging signal RNA secondary structure predicted using Mfold. mSD, major splice donor, PBS, primer binding site. (B) Schematic representation of full length MMTV genome, showing the region used to create the T7 expression plasmid, SA035. (C) SHAPE-constrained RNA structure model of MMTV packaging signal. Nucleotides are color annotated as per the SHAPE reactivities key depending on their modification by BzCN. The data shown is an average of 3-6 independent experiments.