Figure 4

VifB3 mutant encapsidation into HIV particles is defective. 293T cells were cotransfected with A3G or HAA3G22K and a proviral construct HXB2N, HXB2NΔVif or HXB2B3. At 48 h post-transfection, cells and culture supernatants were harvested, and viral particles were precipitated by ultracentrifugation for Western blot analysis (A). HXB2N, HXB2NΔVif or HXB2B3 was transfected into 293T cells. At 48 h post-transfection, cells were collected, and viral particles in culture supernatants were precipitated by ultracentrifugation for Western blot analysis (B). The percentage represents the quantity of intraviral Vif normalized by the quantity of intraviral p24. HXB2N was set to 100%.