Figure 3

Vif counteracts the ability of A3G to induce G to A hypermutations. HXB2N, HXB2NΔVif or HXB2B3 was cotransfected with A3G, HAA3G22K or A3GD128K into 293T cells. At 48 h post-transfection, culture supernatants were harvested, and virus particles were precipitated by ultracentrifugation for Western blot analysis (A). The cell culture supernatants were also used to infect TZM-bl indicator cells to measure viral infectivity (B). The cell culture supernatants from panel A were used to infect SupT1 cells for hypermutation analysis (C).