Figure 5From: Triptolide inhibits human immunodeficiency virus type 1 replication by promoting proteasomal degradation of Tat proteinTriptolide specifically reduces steady-state level of Tat protein. (A) HeLa cells were transfected with pEF1-FLAG-Tat in the presence of increasing concentrations of triptolide. Cells were harvested 48 h post-transfection, and protein expression levels were determined by Western blotting. (B) HeLa cells were transfected either with pEF1-GFP (left) or pEF1-Tat-GFP (right) in the presence of increasing concentrations of triptolide. Cells were harvested 48 h post-transfection, and protein expression levels were determined by Western blotting.Back to article page