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Table 2 Env properties on high CD4 cells

From: Resistance to N-peptide fusion inhibitors correlates with thermodynamic stability of the gp41 six-helix bundle but not HIV entry kinetics

Resistance pathway

Construct

Infectivity

 

Kinetics

 

N44 resistance

Relative infectivitya,b

SEM (+/−)

T1/2 (min)c

SEM (+/−)

Relative N44 resistancea,b

SEM (+/−)

WT

WT-WT

1.00

0.28

53.13

0.61

1.04

0.14

 

C1-WT

1.77

0.11

61.83

0.80

1.09

0.07

 

C2-WT

1.69

0.08

61.39

2.10

1.20

0.13

 

C3-WT

1.07

0.10

59.00

1.26

1.10

0.05

 

C4-WT

2.21

0.11

58.91

1.17

1.23

0.03

 

WT-V833Id

1.28

0.44

56.48

1.80

1.04

0.08

 

WT-P714Ld

1.54

0.14

56.68

3.77

1.09

0.12

E560K

WT-E560K

0.40

0.04

58.06

1.59

1.29

0.09

 

WT-C1

0.18

0.07

64.98

0.80

2.50

0.03

 

C1-C1

0.51

0.15

71.13

0.64

2.37

0.03

 

C4-C1

0.22

0.08

69.36

0.72

2.34

0.19

 

WT-C2

0.73

0.20

66.98

2.50

2.26

0.30

 

C2-C2

0.39

0.01

72.00

0.41

1.58

0.05

 

C3-C2

0.83

0.30

66.69

3.12

2.14

0.07

 

WT-C6

0.43

0.06

67.36

1.90

3.40

0.11

 

C6-C6

0.38

0.00

65.54

2.19

3.22

0.11

Dual

C2-KKR

0.53

0.16

68.54

2.28

1.61

0.23

 

C4-KKR

0.29

0.04

67.66

0.85

1.87

0.28

 

WT-KKR

0.60

0.26

64.92

1.50

2.10

0.03

E648K

WT-C3

1.40

0.14

48.82

0.60

1.88

0.02

 

C3-C3

1.23

0.36

54.41

1.85

1.84

0.21

 

C2-C3

1.75

0.05

64.62

3.90

1.50

0.03

 

WT-C4

2.35

0.65

52.92

1.35

3.59

0.16

 

C4-C4

1.27

0.29

58.06

1.34

3.86

0.30

 

C1-C4

0.81

0.20

65.18

1.76

2.52

0.04

 

C4-C4-Te

1.23

0.39

61.33

1.91

3.39

0.42

 

C5-C5

0.15

0.05

69.73

4.70

0.96

0.06

 

C5-C5-Te

0.26

0.06

70.28

2.30

0.90

0nnn12

  1. aPerformed on U87-CD4-CCR5 cells.
  2. bNormalization was performed against a different WT pseudovirus lot for each independent experiment.
  3. cPerformed on JC53 cells.
  4. dMutation in C-terminal tail.
  5. eT indicates that the C-terminal tail mutation was removed.