Resistance to N-peptide fusion inhibitors correlates with thermodynamic stability of the gp41 six-helix bundle but not HIV entry kinetics

Table 1 Resistance mutations in Env constructs

Env category	Pathway	Construct name^a	gp120 mutations	gp41 mutations
				HR1	HR2	other^b
Selected	E560K	C1-C1	L125F, I165K	N554K, E560K, V580L	T641I
		C2-C2	L125F, E429K	E560K, Q577R
		C6-C6	V169G	E560K, Q577R	T641I
	E648K	C3-C3	T319I		E648K
		C4-C4	T319I, E322D, S306G	Q577R	T641I, E648K	D620N, V833I
		C5-C5	A221V, I309M, D167N		E648K	P714L
Chimeric	E560K	WT-C1		N554K, E560K, V580L	T641I
		C4-C1	T319I, E322D, S306G	N554K, E560K, V580L	T641I
		WT-C2		E560K, Q577R
		C3-C2	T319I	E560K, Q577R
		WT-C6		E560K, Q577R	T641I
		E560K			E560K
	E648K	WT-C3			E648K
		C2-C3	L125F, E429K		E648K
		WT-C4		Q577R	T641I, E648K	D620N, V833I
		C1-C4	L125F, I165K	Q577R	T641I, E648K	D620N, V833I
Dual		KKR		E560K, Q577R	E648K
		C2-KKR	L125F, E429K	E560K, Q577R	E648K
		C4-KKR	T319I, E322D, S306G	E560K, Q577R	E648K
C-Tail mutations		C4-C4-T	T319I, E322D, S306G	Q577R	T641I, E648K	D620N
		WT-V833I				V833I
		C5-C5-T	A221V, I309M, D167N		E648K	P714L
		WT-P714L				P714L
WT gp41 constructs		C1-WT	L125F, I165K
		C2-WT	L125F, E429K
		C3-WT	T319I
		C4-WT	T319I, E322D, S306G

^aConstruct named by donor gp120-donor gp41. For example, C1-C4 represents the gp120 from culture C1 and the gp41 from culture C4.
^bMutations outside of 6HB region.

ISSN: 1742-4690