Figure 7
From: Neutralisation of HIV-1 cell-cell spread by human and llama antibodies
Neutralisation of cell-cell spread from macrophages to T cells by J3 and J3-Fc. HIV-1 (BaL) infected macrophages were incubated with the indicated concentrations of (A) J3, (B) J3-Fc and negative controls (Lab5 or B6) for 1 h at 37°C. An equal number of autologous, uninfected CD4+ T cells were then added and cells incubated for 48 h. T cells were recovered from macrophages by washing, fixed and stained for intracellular HIV-1 Gag and analysed by flow cytometry. The percentage of Gag + T cells relative to controls (Lab5 for J3 and B6 for J3-Fc) is shown. Data are the mean and SEM from 3 independent experiments. Statistical analysis was performed by Anova with Tukey's post-test.