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Table 1 Aggregation assessment of trimeric Env or HIV-1 BaL virions incubated with antibody

From: Aggregate complexes of HIV-1 induced by multimeric antibodies

Assessment of IgG antibodies for aggregation of gp140 and HIV-1
Antibody Epitope (residues) Trimeric CN54 gp140 Neutralization Purified HIV-1 BaL
gp140 Agg.1 Conc. range2 Ab Conc. Ab:Env ratio PDI3 IC50 Virus Agg.1 Δ Virus size4(nm) PDI3
HIV-Ig Polyclonal ++ 50-1 50 17:1 0.67 10.6 - 19.3 0.41
1B7 gp41 (ND) ++ 50-10 10 3.4:1 0.56 >50 - 5.4 0.23
5F3 gp41 cluster V ++ 50-2 5 1.7:1 0.53 >50 - 12.5 0.30
24G3 gp41 (526-543) ++ 50-5 5 1.7:1 0.49 >50 - 10.6 0.26
3H12 gp41 (ND5) ++ 50-.5 2 1:1.5 0.45 >50 - 4.0 0.33
b12 gp120 CD4bs ++ 50-2 5 1.7:1 0.54 0.75 - 12.7 0.34
G8 gp120 (dis) ++ 5-2 5 1.7:1 0.51 >50 - -6.4 0.21
1F7 gp120 V3 ++ 50-1 5 1.7:1 0.48 1.24 - 6.3 0.32
25C2 gp41 CD4i (526-543) + 50-5 10 3.4:1 0.35 >50 - -1.6 0.34
1H5 gp41 (579-613) + 50-2 2 1:1.5 0.32 >50 - 1.0 0.23
4B3 gp41 (579-613) + 50-5 5 1.7:1 0.31 >50 - -4.3 0.28
1B1 gp120 (CD4bs dis) + 50-10 10 3.4:1 0.37 >50 - 7.4 0.27
3D5 gp120 (ND) + 50-5 5 1.7:1 0.34 >50 - -12.4 0.21
2G6 gp120 (CD4bs dis) + 10-5 10 3.4:1 0.32 >50 - 1.4 0.26
3B7 gp120 (non-V3) + 50-5 10 3.4:1 0.28 >50 - 12.6 0.31
4D4 gp41 (579-613) - 10 10 3.4:1 0.30 >50 - 16.2 0.28
1F11 gp41 (579-613) + 10 10 3.4:1 0.27 >50 - 2.2 0.40
3D6 gp41 (604-617) ± 50-5 5 1.7:1 0.23 >50 - 9.8 0.27
2G12 gp120 (C2-C3-V4 glycan) ± 50-10 10 3.4:1 0.19 1.89 - 13.7 0.26
7B2 gp41 cluster I ± 50 50 17:1 0.27 9.65 - 11.0 0.38
2F5 gp41 MPER (662-667) - N/A N/A N/A 0.21 2.73 - 14.6 0.37
4E10 gp41 MPER (824-830) - N/A N/A N/A 0.20 >50 - -1.5 0.22
  1. The average size of the preparations was measured by dynamic light scattering. Data is the average of three experiments, each measured in triplicate. For CN54gp140, Ab concentration, Ab:Env ratio and PDI are reported for optimal aggregate formation conditions. For HIV-1 BaL, Δ Virus size and PDI are reported for 10 μgml-1. All concentrations are in μgml-1.
  2. 1Aggregate formation was scored by the maximum size measured: ++ for particles > 100 nm; + for 40 ≤ 100 nm; ± for 20 < 40 nm, - for ≤ 20 nm.
  3. 2Concentration range where the gp140-Ab complexes scored positive for aggregation.
  4. 3Relative heterogeneity of preparations is evaluated by polydispersity index (PDI).
  5. 4Change from purified virus particle size of 150.2 nm.
  6. 5ND: not determined.