Table 1 Aggregation assessment of trimeric Env or HIV-1 BaL virions incubated with antibody
From: Aggregate complexes of HIV-1 induced by multimeric antibodies
Assessment of IgG antibodies for aggregation of gp140 and HIV-1 | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
Antibody | Epitope (residues) | Trimeric CN54 gp140 | Neutralization | Purified HIV-1 BaL | ||||||
gp140 Agg.1 | Conc. range2 | Ab Conc. | Ab:Env ratio | PDI3 | IC50 | Virus Agg.1 | Δ Virus size4(nm) | PDI3 | ||
HIV-Ig | Polyclonal | ++ | 50-1 | 50 | 17:1 | 0.67 | 10.6 | - | 19.3 | 0.41 |
1B7 | gp41 (ND) | ++ | 50-10 | 10 | 3.4:1 | 0.56 | >50 | - | 5.4 | 0.23 |
5F3 | gp41 cluster V | ++ | 50-2 | 5 | 1.7:1 | 0.53 | >50 | - | 12.5 | 0.30 |
24G3 | gp41 (526-543) | ++ | 50-5 | 5 | 1.7:1 | 0.49 | >50 | - | 10.6 | 0.26 |
3H12 | gp41 (ND5) | ++ | 50-.5 | 2 | 1:1.5 | 0.45 | >50 | - | 4.0 | 0.33 |
b12 | gp120 CD4bs | ++ | 50-2 | 5 | 1.7:1 | 0.54 | 0.75 | - | 12.7 | 0.34 |
G8 | gp120 (dis) | ++ | 5-2 | 5 | 1.7:1 | 0.51 | >50 | - | -6.4 | 0.21 |
1F7 | gp120 V3 | ++ | 50-1 | 5 | 1.7:1 | 0.48 | 1.24 | - | 6.3 | 0.32 |
25C2 | gp41 CD4i (526-543) | + | 50-5 | 10 | 3.4:1 | 0.35 | >50 | - | -1.6 | 0.34 |
1H5 | gp41 (579-613) | + | 50-2 | 2 | 1:1.5 | 0.32 | >50 | - | 1.0 | 0.23 |
4B3 | gp41 (579-613) | + | 50-5 | 5 | 1.7:1 | 0.31 | >50 | - | -4.3 | 0.28 |
1B1 | gp120 (CD4bs dis) | + | 50-10 | 10 | 3.4:1 | 0.37 | >50 | - | 7.4 | 0.27 |
3D5 | gp120 (ND) | + | 50-5 | 5 | 1.7:1 | 0.34 | >50 | - | -12.4 | 0.21 |
2G6 | gp120 (CD4bs dis) | + | 10-5 | 10 | 3.4:1 | 0.32 | >50 | - | 1.4 | 0.26 |
3B7 | gp120 (non-V3) | + | 50-5 | 10 | 3.4:1 | 0.28 | >50 | - | 12.6 | 0.31 |
4D4 | gp41 (579-613) | - | 10 | 10 | 3.4:1 | 0.30 | >50 | - | 16.2 | 0.28 |
1F11 | gp41 (579-613) | + | 10 | 10 | 3.4:1 | 0.27 | >50 | - | 2.2 | 0.40 |
3D6 | gp41 (604-617) | ± | 50-5 | 5 | 1.7:1 | 0.23 | >50 | - | 9.8 | 0.27 |
2G12 | gp120 (C2-C3-V4 glycan) | ± | 50-10 | 10 | 3.4:1 | 0.19 | 1.89 | - | 13.7 | 0.26 |
7B2 | gp41 cluster I | ± | 50 | 50 | 17:1 | 0.27 | 9.65 | - | 11.0 | 0.38 |
2F5 | gp41 MPER (662-667) | - | N/A | N/A | N/A | 0.21 | 2.73 | - | 14.6 | 0.37 |
4E10 | gp41 MPER (824-830) | - | N/A | N/A | N/A | 0.20 | >50 | - | -1.5 | 0.22 |