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Figure 2 | Retrovirology

Figure 2

From: Role of cullin-elonginB-elonginC E3 complex in bovine immunodeficiency virus and maedi-visna virus Vif-mediated degradation of host A3Z2-Z3 proteins

Figure 2

BIV Vif recruits Cul2-EloB-EloC and MVV Vif recruits Cul5-EloB-EloC to induce btA3Z2-Z3 and oaA3Z2-Z3 degradation. 293 T cells (5 × 106) were transfected with 10 μg HA-tagged BIV Vif or 10 μg VHL and 10 μg VR1012 as a positive and a negative control, respectively (A, B); or with 10 μg HA-tagged MVV Vif or 10 μg HIV Vif and 10 μg VR1012 as a positive and a negative control, respectively (C, D). After 48 h of transfection, cell lysates were immunoprecipitated with HA beads, followed by SDS-PAGE and immunoblot analysis using anti-HA, anti-hCul2, anti-hCul5, anti-hEloB, anti-hEloC and anti-hCBF-β antibodies. All immunoprecipitation experiments were repeated three times.

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