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Figure 8 | Retrovirology

Figure 8

From: Partial rescue of V1V2 mutant infectivity by HIV-1 cell-cell transmission supports the domain’s exceptional capacity for sequence variation

Figure 8

V1V2 function analysis of strains SF162 and P3N. (A) Activity of SF162 and P3N wt and mutant envs was compared in free virus infection of TZM-bl cells (black) and cell-cell transmission from 293-T cells to A3.01-CCR5 cells (red). Data are normalized to SF162 wt. (B) Comparison of the same virus panel shown in (A) in free virus infection of TZM-bl cells (black) and cell-cell fusion activity between 293-T cells and TZM-blrhTRIM5α cells (green). Data are normalized to SF162 wt. (C) Free virus neutralization of env-pvNLLuc pseudoviruses by anti-V3 mAb 1.79 (right) and anti-CD4bs mAb b6 (left). (D) Assessment of virus temperature stabilities upon long-term incubation of P3N and SF162 wt and ΔV1V2 virus stocks. (E) Assessment of virus temperature stabilities upon temperature gradient incubation of P3N and SF162 wt and ΔV1V2 virus stocks. (A) to (E): All data shown are mean and SD from 2 to 3 independent experiments performed in duplicates.

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