Figure 2

JR-FL V2 mutations decrease cell-cell transmission capacity less than free virus infectivity. (A) JR-FL V2 point mutant infectivities were normalized to JR-FL wt for both free virus infection (black) and cell-cell transmission (red). Values of relative efficacy of cell-cell transmission versus free virus infection are shown below the bars; a star indicates whether this difference is statistically significant as probed by multiple unpaired t-tests with alpha = 0.05. Mean and SD from 4 independent experiments performed in duplicates are depicted. (B) Analysis of JR-FL wt and V2 point mutants in T-cell to T-cell transmission using Jurkat donor cells (expressing replication competent TN6^inGLuc reporter viruses) and A301-CCR5 target cells (orange). Cell-cell transmission activity was compared to free virus infectivity of the same Jurkat derived viruses on TZM-bl cells (black). Data are normalized to JR-FL wt. Values of relative efficacy of cell-cell transmission versus free virus infection are shown below the bars; a star indicates whether this difference is statistically significant as probed by multiple unpaired t-tests with alpha = 0.05. Mean and SD from 2 independent experiments performed in duplicates are depicted. (C) Analysis of JR-FL wt and V2 point mutant free virus infectivity (black) and cell-cell fusion capacity (green). Data are normalized to JR-FL wt. Values of relative efficacy of cell-cell fusion versus free virus infection are shown below the bars; a star indicates whether this difference is statistically significant as probed by multiple unpaired t-tests with alpha = 0.05. Mean and SD from 2 independent experiments performed in duplicates are depicted.