Figure 2

High frequency HIV-1 specific T cell responses were induced in acute infection in CH078. Ex vivo IFN-γ ELISpots were performed on PBMCs from CH078 between 21 and 442 days post-screening (Fiebig I/II). Solid colored lines represent individual T cell responses and are plotted as absolute magnitude (A) or % of total magnitude (B). The plasma virus load (pVL) of CH078 is plotted as a dotted line on each graph. A T cell response was considered positive if it was ≥30 SFU/10⁶ cells and ≥4× background. All data represent the background subtracted mean spot forming units (SFU) per million PBMCs. For each response the epitope name based on HXB2 aligned amino acid position within individual proteins, the experimentally confirmed sequence, HLA restriction and epitope entropy are listed below the figure. Parentheses around HLA types indicate the HLA type predicted for the epitope based on binding motif as opposed to experimentally confirmed HLA restriction.