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Figure 5 | Retrovirology

Figure 5

From: NUCKS1, a novel Tat coactivator, plays a crucial role in HIV-1 replication by increasing Tat-mediated viral transcription on the HIV-1 LTR promoter

Figure 5

Key role of NUCKS1 in viral production and replication. (A-B) In the single-round replication assay, HeLa cells were transfected with control siRNA or three individual NUCKS1 siRNAs. One day later, the cells were transfected with pNL4-3 (A) or pAD8 (B) proviral DNA. Two days after siRNA transfection, the virus-containing supernatants were harvested every day for 5 days. The amount of virion in the supernatant was measured by HIV-1 p24 ELISA. (C) In the viral replication assay, Jurkat and A3.01 cells were transfected with control siRNA or three individual NUCKS1 siRNAs. One day later, the cells were infected with pNL4-3 virus. Five days after infection, the virus-containing supernatants were harvested, and then the amount of virions in the supernatant was measured by HIV-1 p24 ELISA. The data are expressed as mean ± SD (n = 3). *, P < 0.01 as compared with the cells transfected with control siRNA. The lower panels show the intracellular p24 levels.

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Retrovirology

ISSN: 1742-4690

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