Figure 5

V1V2 loop length and N-linked glycosylation (CSF vs Plasma). The mean (A) amino acid lengths and (B) number of potential N-linked glyosylation sites (PNLGS) of the HIV-1 env V1V2 region from paired CSF and plasma quasispecies are shown for all subjects (n = 15). (C) Linear regression of the mean amino acid lengths of the HIV-1 env V1V2 region from CSF quasispecies with the corresponding mean number of PNLGS in the same quasispecies. (D) The mean amino acid lengths of the HIV-1 env V1V2 region in the CSF quasispecies of individuals with NCN and those with ANI + MND. The Wilcoxon matched-pairs signed rank test and the Mann Whitney test were used to determine p-values for paired observations (CSF vs. plasma) and observations between disease classifications (NCN vs ANI + MND) respectively. The linear regression score (r²) was derived in PRISM. For all comparisons, p-values <0.05 are considered significant. * = Statistically significant.