Figure 3

Faster transduction in dual Vpx + VLP treated MDMs. (A) Diagram showing the experimental procedure for single VLP treatment of MDMs. VLP and D3HIV treatments occurred at day 0, followed by collecting the cells for FACS analysis at days 1, 2 and 7. (B) An illustration depicting the experimental design with the dual VLP treatments occurring at days 0 and 7. D3HIV treatment was done at day 7. Cells were collected at days 8, 9 and 14, corresponding to days 1, 2 and 7, respectively, after D3HIV vector addition to the medium. (C-D) FACS analysis monitoring the frequency of GFP + MDMs for two independent donors treated with single VLP: Vpx- (white bars), Vpx + (black bars) or dual VLP: Vpx- (yellow bars) and Vpx + (red bars). Significant differences were determined first using one-way ANOVA, followed by comparing each group using Mann–Whitney analysis. ***denotes P < 0.001.