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Table 6 Inhibition of HIV-1 NL4.3 capture and transmission* by Raji/DC-SIGN cells, quantified by p24-ELISA

From: A phenyl-thiadiazolylidene-amine derivative ejects zinc from retroviral nucleocapsid zinc fingers and inactivates HIV virions

Compound

IC50(μM)

HIV-capture procedure A

Transmission procedure B

Transmission procedure C

WDO-217

>105

2.2 ± 1

8.3 ± 0.4

HHA

0.014 ± 0.01

0.002 ± 0.001

≤0.003

  1. *In co-cultivation with C8166 cells; samples examined after 42 hours of incubation.
  2. Procedure A: HIV was pretreated (1 h) with compound before it was added to Raji/DC-SIGN cells. Capture was measured by p24 analysis of the washed cell pellet.
  3. Procedure B: Raji/DC-SIGN cells were exposed to compound-pretreated (1 h) HIV after which compound was removed and the cells were cocultured in the presence of C8166 cells. The read-out of this experiment was the replication capacity of virus in C8166 as monitored by p24 analysis.
  4. Procedure C: HIV-captured Raji/DC-SIGN cells were cocultivated with C8166 cells in the presence of compound (microscopic measurement of giant cell formation).
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Retrovirology

ISSN: 1742-4690

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