Figure 6
Inhibition of NCp7(11-55)-induced cTAR destabilization by WDO-217. (A) Emission spectra of Rh6G-cTAR-Dabcyl (0.1 μM) recorded in the absence (circle) or in the presence of NCp7(11-55) (1 μM) (square). To determine the importance of the order of addition of the compounds, the emission spectra of Rh6G-cTAR-Dabcyl (0.1 μM) were recorded either after addition of NCp7(11-55) preincubated with 10 μM WD0-217 (open triangle) or after preincubation with 10 μM WDO-217 and then, addition of 10 μM NCp7(11-55) (closed triangles). Excitation wavelength was 520 nm. (B) Inhibition kinetics of NC-induced cTAR destabilization by WDO-217. Emission spectra of Rh6G-cTAR-Dabcyl (0.1 μM) were recorded in the absence (circle) and in the presence of NCp7(11-55) (1 μM) (square) and at different times after addition of WDO-217 (10 μM) as indicated. Excitation wavelength was 520 nm. (C) Correlation between the kinetics of Zn2+ ejection and inhibition of NC-induced cTAR destabilization by WDO-217 (10 μM). Inhibition of cTAR destabilization (triangle) correlates well with zinc ejection (star).