Skip to main content
Figure 3 | Retrovirology

Figure 3

From: A phenyl-thiadiazolylidene-amine derivative ejects zinc from retroviral nucleocapsid zinc fingers and inactivates HIV virions

Figure 3

Effects of WDO-217 on virion-associated Gag p24 CA and genomic mRNA content of HIV-1 virus particles. Isolated HIV-1 particles were incubated with different concentrations of compounds and subsequently cleared from excess compound by multiple washing and centrifugation steps. (A) The amount of virion-associated p24 was quantified by ELISA, and the total virus-associated genomic RNA was quantified by RT-qPCR. (B) Genomic RNA of untreated virus (1) or virus treated with 0.5% Triton X-100 (2) or 125 μM WDO-217 (3) was visualized by Northern blot. (C) Dose dependent decrease of genomic RNA in treated virus particles as quantified by RT-qPCR. Concentration of compound used is given between brackets. Results are mean ± std dev with n = 3.

Back to article page

Retrovirology

ISSN: 1742-4690

Contact us