Identification of novel CDK9 and Cyclin T1-associated protein complexes (CCAPs) whose siRNA depletion enhances HIV-1 Tat function

Table 1 Protein subunits of CCAPs depleted in this study

Protein depleted	CCAP	Name	Other names	Function of depleted protein	Reference
FBXO11	FBXO11	F-box protein 11	UBR6;VIT1; FBX11; PRMT9; UG063H01	Phosphorylation-dependent ubiquitination, regulates p53 and BCL6	[29, 30]
	CUL1	Cullin 1	-
	SKP1	S-phase kinase-associated protein 1	OCP2; p19A; EMC19; SKP1A; OCP-II; TCEB1L
PPP1R10		protein phosphatase 1, regulatory subunit 10	FB19; CAT53; PNUTS; PP1R10	Targets protein phosphatase-1 (PP1) to the nucleus, in DNA damage response	[31]
WDR82	PNUTS	WD repeat domain 82	SWD2; MST107; WDR82A; MSTP107; PRO2730; TMEM113; PRO34047	Component of the mammalian SET1A /SET1B histone H3-Lys4-methyltransferase complexes	[32]
TOX3	PNUTS	TOX high mobility group box family member 3	CAGF9; TNRC9	Unwinding of DNA, chromatin structure alteration and neuronal transcription	[33]
TPR	TPR	Translocated promoter region (to activated MET oncogene)		Directly interacts with several components of nuclear pore complexes (NPCs). Nuclear export of mRNAs and some proteins.	[34]
TTF2	TTF2	Transcription termination factor, RNA polymerase II	HuF2	Critical role in altering protein-DNA interactions. Has dsDNA-dependent ATPase and RNAPII termination activity. Plays a role in pre-mRNA splicing.	[35, 36]
WRNIP1	WRNIP1	Werner helicase interacting protein 1	WHIP; bA420G6.2; RP11-420G6.2	Interacts with the N-terminal portion of Werner protein containing the exonuclease domain and implicated in Werner’s syndrome. It may influence aging process.	[37–39]

The proteins that were depleted using siRNA in this study are listed. The core complex these proteins exist in cells as presented in Figure 1 is included along with brief description of their cellular function.

ISSN: 1742-4690