Figure 3

Neutralization of pseudovirions carrying primary HIV-1 envelopes with and without mutated W100 pockets. For AD8, JR-CSF and JR-FL, a single substitution introduced R373 to combine with the N386 glycan already present in these envelopes to form the R373/N386 glycan combination to block the W100 pocket. The wild type LN40 envelope already carries the R373/N386 glycan combination and was mutated to change both these residues to K373/D386. The reciprocal substitutions in B33 converted K373/D386 to R373/N386. All envelopes carrying a putative closed W100 pocket were substantially more resistant to mab b12, but remained sensitive to VRC01 and VRC03. Some modest shifts in sensitivity were observed and are discussed in the main text. Each data point shown was obtained from averaging readings obtained from two independent neutralization tests each with duplicate points.