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Figure 1 | Retrovirology

Figure 1

From: Impact of immune escape mutations on HIV-1 fitness in the context of the cognate transmitted/founder genome

Figure 1

Selection of CTL escape mutations in the HIV-1-infected subject. (A) Frequencies of mutations in the TW10 CTL epitope at screening and later time points (days post screening) were determined by SGA [11, 29]. The positions of amino acid substitutions are indicated above the TW10 epitope sequence. (B) T cell response to WT and mutant TW10 peptides (Gag240-249) at day 592 were determined using an ex vivo IFN-γ ELISpot assay. Positive T cell responses were defined as: ≥ 30 SFU/million and > 4 times above background. All assays were performed in duplicate. HLA typing and T cell mapping using autologous peptides spanning the transmitted founder virus was previously described [11]. (C) Frequencies of the viruses with I64T and/or R355K mutations at screening and later time points were determined by SGA [11, 29]. The TK virus contains both I62T and R355K mutations.

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