Figure 1

CD4 T lymphocytes express SAMHD1. a. Western blot analysis of SAMHD1 expression in primary hematopoietic cells. b. Flow cytometry analysis of SAMHD1 expression levels in CD14⁺ monocytes, T-cells (CD4⁺ and CD8⁺ T lymphocytes) and in CD4⁺ T-cell subsets including Naïve (Tn; CD45RA⁺, CCR7⁺), Central Memory (Tcm; CD45RA^-, CCR7⁺), and Effector Memory (Tem; CD45RA^-, CCR7^-). c. Kinetics of SAMHD1 loss in quiescent (CD69^-, HLA-DR^-) CD4⁺ T-cells after VLP-Vpx treatment (representative experiment, n = 2). d. Vpx transduction of activated CD4⁺ T-cells induces loss of SAMHD1. TCR-stimulated CD4⁺ T-cells were transduced with Vpx-HA expressing retroviral construct. Forty-eight hours later, SAMHD1 and Vpx were immunostained using specific antibodies. Nuclei were stained in mounting media with DAPI. Pie charts represent the proportions of SAMHD1⁺ cells among 100 Vpx positive and negative counted cells.